<p style="text-align: center;">WANG He, YANG Fangyuan, ZHAN He, GUO Qiulei, HONG Xinxun , CUI Zhiyuan, LIU Qingguo</p><p style="text-align: center;">( School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029,&nbsp;<span style="text-align: center;">China )</span></p><p style="text-align: justify;"><strong>Abstract</strong>：<strong>Objective</strong>: To explore the effect difference between twirling-rotating reinforcing and reducing technique of acupuncture on Cerebellum NE and 5-HT in Spontaneously Hypertensive rats (SHR) andits protectivemechanism.Methods:&nbsp; Fifty male 9-week-old SHR rats were randomly&nbsp; divided into 4 groups: normal&nbsp; group,&nbsp; model&nbsp; group,&nbsp; needle&nbsp; retaining&nbsp; group,&nbsp; twirling-rotating&nbsp; reinforcing&nbsp; group,twirling-rotating reducing group, 10 per group.Acupuncture was not used in normal and control group,only with grasp,capture and binding&nbsp; stimulation that&nbsp; was&nbsp; also adapt&nbsp; in the&nbsp; rest&nbsp; groups.&nbsp; Needle&nbsp; retaining group was&nbsp; treated&nbsp; with acupuncture&nbsp; at&nbsp; “Taichong“&nbsp; (LR3&nbsp; )&nbsp; without&nbsp; any&nbsp; technique&nbsp; and&nbsp; retaining&nbsp; for&nbsp; 10&nbsp; min&nbsp; .Twirling-rotating reinforcing group and twirling-rotating reducing group were treated with Twirling-rotating reinforcing and reducing techniques&nbsp; respectivelyat&nbsp; &nbsp;(LR3 ) and retaining for 10 min. Before and after acupuncture, blood pressure&nbsp; monitor&nbsp; was&nbsp; used&nbsp; to&nbsp; measure&nbsp; the&nbsp; blood&nbsp; systolic&nbsp; pressure.&nbsp; 28&nbsp; days&nbsp; after&nbsp; treatment,&nbsp; taking&nbsp; the cerebellum,&nbsp; Elisa was used to detect norepinephrine and serotonin in&nbsp; the cerebellum. <strong>Results</strong>:&nbsp; The systolic blood pressure of the model rats wassignificantly higher than that of the normal group&nbsp; (P&lt;0.05). Since 3 days&nbsp; after&nbsp; treatment,the&nbsp; blood&nbsp; pressure&nbsp; in&nbsp; needle&nbsp; retaining&nbsp; group,&nbsp; twirling-rotating&nbsp; reinforcing group,twirling-rotating&nbsp; reducing&nbsp; groupdecreased&nbsp; than&nbsp; that&nbsp; of&nbsp; model&nbsp; group&nbsp; (P&nbsp; &lt;0.05);Compared&nbsp; with&nbsp; the normal group, the content of NE and 5&nbsp; -&nbsp; HT in the cerebellum of model group, needle retaining group, twirling-rotating&nbsp; reinforcing&nbsp; group,twirling-rotating&nbsp; reducing&nbsp; groupwere&nbsp; significantly&nbsp; increased&nbsp; (P &lt;0.05);Compared&nbsp; with&nbsp; model&nbsp; group,&nbsp; the&nbsp; contents&nbsp; of&nbsp; NE&nbsp; and&nbsp; 5-HT&nbsp; in&nbsp; needle&nbsp; retaining&nbsp; group,&nbsp; twirlingrotating&nbsp; reinforcing&nbsp; group,twirling-rotating&nbsp; reducing&nbsp; group&nbsp; were&nbsp; significantly&nbsp; decreased&nbsp; (P &lt;0.05);Compared with needle and twirling-rotating reinforcing group,twirling-rotating reducing group NE decreased&nbsp; significantly&nbsp; (P&lt;0.05). <strong>Conclusion</strong>:&nbsp; Acupuncture&nbsp; twirling-rotating&nbsp; reinforcing&nbsp; and&nbsp; reducing technique can effectively reduce the systolic blood pressure of SHR rats. The antihype rtensive effect of acupuncture&nbsp; and&nbsp; diarrhea&nbsp; was&nbsp; related&nbsp; to&nbsp; the&nbsp; contents&nbsp; ofnorepinephrine(NE)&nbsp; and&nbsp; 5-HT&nbsp; (5-HT),&nbsp; and&nbsp; the regulation effect of twirling-rotating reinforcing and reducing technique were different.</p><p><br/></p>