<p style="text-align: center;">YANG Zhixin, LIU Dingding,QU Jingyang,AN Na,XU Qian, CHEN Long</p><p style="text-align: center;">(1 Chengde Medical University, Chengde Hebei 067000; 2&nbsp; North China University of Science and Technology, Tangshan&nbsp; Hebei&nbsp; 063000;&nbsp; 3Hebei&nbsp; Province&nbsp; Key&nbsp; Laboratory&nbsp; of&nbsp; Research&nbsp; and&nbsp; Development&nbsp; for&nbsp; Chinese Medicine, Chengde Medical University, Chengde Hebei 067000)</p><p style="text-align: justify;"><strong>Abstract</strong>:&nbsp; <strong>Objective</strong>:&nbsp; To&nbsp; observe&nbsp; the&nbsp; expression&nbsp; of&nbsp; substance&nbsp; P&nbsp; in&nbsp; the&nbsp; point&nbsp; local&nbsp; and&nbsp; serum&nbsp; after acupuncture the&nbsp; “Complementary Acupoints”&nbsp; Yanglingquan and Yinlingquan in rats, so as to analyze the correlation&nbsp; with&nbsp; the&nbsp; change&nbsp; of&nbsp; substance&nbsp; P&nbsp; and&nbsp; the&nbsp; synergistic&nbsp; interaction&nbsp; of&nbsp; compatibility&nbsp; in “Complementary Acupoints”.&nbsp; <strong>Methods</strong>:&nbsp; 40 SD rats were randomly and equally divided into 5 groups,8 rats each.&nbsp; Detection the content ofsubstance P in ratsserum by ELISA technique and the expression of substance P in the point local by histochemistry method after electroacupuncture. <strong>Results</strong>: Compared with the group&nbsp; control, the positive expression of SP in the local area and the content of the serum in&nbsp; other groups were increased in different degrees. Compared with all of acupuncture groups, the&nbsp; SP expression was the least in the group of acupuncture the&nbsp; sideward&nbsp; of Yanglingquan and Yinlingquan.&nbsp;<strong>Conclusion</strong>:As compared with other groups, the positive expression of SP in the local area and the content of the serumwere significantly increased .It is one of the possible factors for the synergistic interaction ofcompatibility in “Complementary Acupoints” .</p><p><strong>Key words</strong>: Acupuncture;Complementary Acupoints;Adjunct Points;SP expression</p><p><br/></p>