<p style="text-align: center;">WEI Xu, HAN Jun-ping, YU Yue-yue, LI Shuang, ZHAO Ye, Y ANG Jin-hua, CUI Jian-mei</p><p style="text-align: center;">（1 North China University of Science and Technology, HebeiTangshan 063000）</p><p><strong>Abstract</strong>: <strong>Objective</strong>:&nbsp; To explore the effects of three-acupoint and five-needling method on Eotaxin and RANTES expressions in lung tissues of asthmatic rats. Methods:&nbsp; Thirty SPF male SD rats were randomly divided into blank&nbsp; group,&nbsp; model&nbsp; group&nbsp; and&nbsp; acupuncture&nbsp; group&nbsp; with&nbsp; 10&nbsp; rats&nbsp; in&nbsp; each&nbsp; group&nbsp; with&nbsp; corresponding interventions. Physiological recorder was used to record airway resistance and pulmonary compliance, HE staining was applied to observe the morphologic changes of lung tissues, the immunohistochemical method was applied to test the expression of Eotaxin and RANTES in lung tissues. Results: Compared with the blank group, the airway resistance was higher in the model group(P＜0.05),compared with the model group, the acupuncture&nbsp; group&nbsp; reduced&nbsp; (P ＜ 0.05).&nbsp; Compared&nbsp; with&nbsp; the&nbsp; blank&nbsp; group,&nbsp; the&nbsp; pulmonary&nbsp; compliance reduced in the model group （P＜0.05), compared with the model group, the acupuncture group improved(P＜0.05). HE staining of rat lung indicated that the pathological changes of acupuncture group was obvious, inflammatory reaction was reduced after acupuncture. Compared with&nbsp; the blank group, the expression of Eotaxin&nbsp; and&nbsp; RANTES&nbsp; in&nbsp; lung&nbsp; tissues&nbsp; of&nbsp; model&nbsp; group&nbsp; was&nbsp; higher （P&lt;0.05),&nbsp; the&nbsp; acupuncture&nbsp; group&nbsp; was reduced(P&lt;0.05).&nbsp; Conclusion:&nbsp; Three-acupoint and five-needling method could reduce Eotaxin and RANTES expressions in lung tissues of asthmatic rats, thus reducing airway inflammatory reaction.&nbsp; &nbsp;&nbsp;</p><p><strong>Key words</strong>: acupuncture; bronchial asthma; Eotaxin; RANTES</p>