<p style="text-align: center;">HUANG Yan，WANG Yanping，ZHANG Weibo，JIA Shuyong，LI Hongyan</p><p style="text-align: center;">（1.School of Acu-moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029，China；2.Institute of Acu-moxibustion，China Academy of Chinese Medical Sciences，Beijing 100700，China）</p><p style="text-align: justify;"><strong>Abstract</strong>:&nbsp; <strong>Objective</strong>: To establish a more stable pathological model of blocked channel by ligation in rats.&nbsp;&nbsp;<strong>Methods</strong>:48&nbsp; male&nbsp; SD&nbsp; rats&nbsp; were&nbsp; randomly&nbsp; divided&nbsp; into&nbsp; 4&nbsp; groups&nbsp; (n&nbsp; =&nbsp; 12).&nbsp; The&nbsp; Ren&nbsp; meridian&nbsp; (RN)&nbsp; low resistance line was measured first. Ligation group 1 ligated 1 point on the RN line; ligation group 2 ligated 2 point on the RN line; ligation group 3 ligated 3 point on the RN line; Ligation group 4 ligated 1 point out of the RN line, the ligation width was 1cm, and the ligation interval was 1-2mm. The interstitial fluid pressure wave&nbsp; propagation&nbsp; method&nbsp; was&nbsp; used&nbsp; to&nbsp; measure&nbsp; the&nbsp; amplitude&nbsp; of&nbsp; the&nbsp; interstitial&nbsp; fluid&nbsp; pressure&nbsp; wave propagation&nbsp; of&nbsp; the&nbsp; groups&nbsp; before&nbsp; and&nbsp; after&nbsp; the&nbsp; ligation.&nbsp; &nbsp;Results:The&nbsp; change&nbsp; rate&nbsp; of&nbsp; interstitial&nbsp; fluid pressure&nbsp; wave&nbsp; before&nbsp; and&nbsp; after&nbsp; ligation&nbsp; was&nbsp; 8.17%,&nbsp; -7.83%,&nbsp; -13.27%&nbsp; and&nbsp; -52.80%&nbsp; respectively&nbsp; in&nbsp; four groups.There&nbsp; was&nbsp; no&nbsp; significant&nbsp; difference&nbsp; between&nbsp; the&nbsp; ligation&nbsp; group&nbsp; 4,&nbsp; the&nbsp; ligation&nbsp; group&nbsp; 1&nbsp; and&nbsp; the ligation group 2 after ligation, but the change rate of the three groups increased from positive to negative. The amplitude of the ligation group 3 was significantly reduced (P&lt;0.01) after ligation compared to before ligation. <strong>Conclusion</strong>:The ligation method can establish a stable pathological model of channel blockage.</p><p><strong>Key words</strong>: Meridian;Ligation; Channel Blockage; Rats; Interstitial Fluid Pressure Wave</p><p><br/></p>