<p style="text-align: center;">Li Yanling, Zou Jing, Huang Guofu&nbsp;</p><p style="text-align: center;">(1 Hubei University of Traditional Chinese Medicine，Wuhan 430065，China&nbsp; ；2 Wuhan Hospital of Integrated Chinese and Western Medicine，Wuhan 430022，China；3 WuChang Hospital&nbsp; Affiliated to Wuhan&nbsp;</p><p style="text-align: center;">technology and science University，Wuhan 430063，China)&nbsp;</p><p style="text-align: justify;"><strong>Abstract</strong>:&nbsp;<strong> Objective:</strong> To observe the effect of electroacupuncture at jiaji（EX-B2）&nbsp; on the expression of actin&nbsp; and&nbsp; tubulin&nbsp; in&nbsp; rabbit&nbsp; degenerated&nbsp; intervertebral&nbsp; disc&nbsp; so&nbsp; as&nbsp; to&nbsp; explore&nbsp; its&nbsp; mechanism&nbsp; in&nbsp; relieving intervertebral&nbsp; disc degeneration.&nbsp; Methods: A total of 40 New Zealand rabbits were randomly&nbsp; divided into normal, model, sham operation&nbsp; and EA&nbsp; groups,&nbsp; with 10&nbsp; rabbits in each group. In&nbsp; model group, the&nbsp; lumbar intervertebral disc&nbsp; degeneration&nbsp; was established by using an axial compression device. EA group was treated with EA at bilateral jiaji(EX-B2) for 20 minutes, once daily for 28 days. The expression levels of actin and tubulin&nbsp; of&nbsp; the lumbar&nbsp; intervertebral disc（L4-L5）were assayed by Western blot at the 28th&nbsp; and 56th&nbsp; day after operation. <strong>Results</strong>: The expression levels of actin and tubulin on the postoperative day 28 in the model group&nbsp; and&nbsp; EA group were significantly&nbsp; declined as compared with&nbsp; the normal&nbsp; group&nbsp; and sham&nbsp; operationgroup (P&lt;0.05). The expression levels of actin and tubulin on the postoperative day 56 in the EA group was significantly increased&nbsp; than that on the&nbsp; postoperative&nbsp; day 28 and in the model&nbsp; group&nbsp; on the&nbsp; postoperativeday 56&nbsp; (P&lt;0.05).&nbsp; <strong>Conclusions</strong>: Electroacupuncture&nbsp; at jiaji(EX-B2)&nbsp; could increase the expression levels&nbsp; of actin and&nbsp; tubulin&nbsp; in&nbsp; rabbit degenerated intervertebral disc and delay the intervertebral disc degeneration through promoting the restoration of the cytoskeleton in&nbsp; degenerated intervertebral disc.</p><p><strong>Key words</strong>: intervertebral disc degeneration；electroacupuncture；jiaji（EX-B2）；actin；tubulin</p><p><br/></p>