<p style="text-align: center;">SHI Yin, WU Huangan, SHANG Haixia, ZHOU Jing, LI Tao</p><p style="text-align: center;">(1 Shanghai University of Traditional Chinese Medicine, Shanghai 201203; 2 Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai200030)</p><p><strong>Abstract:</strong> Crohn&#39;s disease is a chronic and recurrent inflammatory bowel disease involving the whole layer of digestive tract. One of its key factors is the increase permeability of intestinal epithelial barrier resulting from&nbsp; abnormality&nbsp; of&nbsp; tight&nbsp; junction&nbsp; pathway&nbsp; of&nbsp; TNF-α-induced&nbsp; NF-κB-MLCK&nbsp; in&nbsp; colonic&nbsp; epithelium.&nbsp; Our previous studies have shown that herbs-partition moxibustion is an effective treatment for Crohn&#39;s disease. This study aims at observing effect of herbs-partition moxibustion in reducing permeability of intestinal epithelial barrier by regulating&nbsp; TNF-α/NF-κB-MLCKtight junction pathway to restore intestinal epithelial barrier and inhibit inflammation in Crohn&#39;s disease.</p><p><strong>Key&nbsp; words</strong>：Crohn&#39;s&nbsp; disease,&nbsp; herbs-partition&nbsp; moxibustion,&nbsp; TNF-α/NF-κB-MLCK,&nbsp; tight&nbsp; junction pathway, vitro study</p><p><br/></p>