<p style="text-align: center;">Y ANG Zhaokun, W ANG Xiaoyu, HE Wei, SHI Hong, SU Yangshuai, HU Ling, JINGXianghong</p><p style="text-align: center;">(Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing ,100700)</p><p style="text-align: justify;"><strong>Abstract</strong>: <strong>Objective</strong>: The aim of this study was to&nbsp; synchronous observe the regulative effect of&nbsp; electroacupuncture&nbsp; (EA)&nbsp; at&nbsp; ST36&nbsp; and&nbsp; CV12&nbsp; on&nbsp; gastrointestinal&nbsp; motility&nbsp; and&nbsp; the&nbsp; firing&nbsp; rate&nbsp; of gastric&nbsp; related neurons in&nbsp; the&nbsp; NTS and DMV after intragastric administration of acid. Methods: 36 rats were randomly divided into three groups: intragastric acid administration group, EA ST36 group and EA CV12 group. Neuronal spikes in the rat brainstem&nbsp; after intragastric administration of HCl(0.5&nbsp; mol/L) was recorded by&nbsp; electrode, which&nbsp; was acutely implanted to span the the nucleus of&nbsp; solitary&nbsp; tract&nbsp; (NTS)&nbsp; and&nbsp; dorsal&nbsp; motor&nbsp; nucleus&nbsp; of&nbsp; vagus&nbsp; (DMV).&nbsp; EGG&nbsp; was&nbsp; continuously monitored and recorded synchronously by the bipolar electrode, which was in the muscle layer of gastric Antrum.&nbsp; Results:&nbsp; Intragastric&nbsp; administration&nbsp; of&nbsp; 0.5M/L&nbsp; HCL&nbsp; led&nbsp; to&nbsp; a&nbsp; rapid&nbsp; increase&nbsp; in integral of gastric slow wave (GSW) and firing rates of excitatory neurons in the NTS, which was significant at 90 seconds and remained elevated for 45mins（P＜0.01）. Acid administration led to a slow decline in&nbsp; firing rates of inhibitory&nbsp; neurons in&nbsp; the&nbsp; DMV that was significant at 4&nbsp; mins and continued to decline for 1 hour（P＜0.01）.&nbsp; In the time-window from 8 mins to 30&nbsp; mins after intragastric acid administration, EA at ST36 and CV12 elicited inhibitory effects on severe gastric motility. There was no difference between EA&nbsp; at&nbsp; ST36 and CV12（P＞0.05）.&nbsp; EA at ST36 and CV12 elicited inhibitory effects on excitatory neurons in NTS with intragastric acid administration, and the inhibition rate induced by EA ST36 significantly increased in comparison with EA CV12（P＜0.05）. EA at ST36 and CV12 did not cause significant changes in firing rates of inhibitory neurons in DMV（P＞0.05）.&nbsp; Conclusion:&nbsp; In conclusion, EA at ST36 and CV12 regulated gastric nociceptive visceral afferent in NTS, rather than visceral motor efferent in DMV in gastric acid challenge. The difference between the two points possibly connected with different never&nbsp; afferent. EA at ST36 directly contacted with NTS through the vagus visceral afferent, and EA at CV12 through sympathetic splanchnic afferent contacted with spinal dorsal horn.</p><p><strong>Key words</strong>:&nbsp; Electroacupuncture, Acupoint, Gastric Acid&nbsp; Challenge，Visceral&nbsp; Sensory , Visceral Motor</p><p><br/></p>